改良抗体结合试验的建立和在灭活狂犬病疫苗效价检测中的应用

摘要

Objective To establish modified antibody binding test (ABT) and investigate it's application in the efficacity test of inactivated rabies vaccines. Methods Modified ABT( M-ABT) was established by the combination of RFFIT and ABT,and refining the dilution of vaccine samples, detection of residual rabies virus neutralizing antibody(RVNA) and calculation of results. The change of the efficacity of ten kinds of inactivated rabies vaccines with different stored periods were detected by MABT. Results MABT could be completed within 28 h,and 2 days were saved compared with ABT. The efficacity detected by MABT was equal to NIH method. Conclusion M-ABT had been successfully established and could be used for the efficacity test of inactivated rabies vaccines.%目的 对抗体结合试验(ABT)进行改进并将其用于灭活狂犬病疫苗效力的测定.方法 将快速荧光灶抑制试验(RFFIT)与ABT方案融合,在疫苗样品稀释、剩余中和抗体检测、结果计算方面进行改进,形成改良抗体结合试验(M-ABT);应用M-ABT对10种灭活狂犬病疫苗随库存时间增加疫苗效力改变的情况进行检测.结果 M-ABT可以在28 h内完成,比ABT节省2 d,检测结果与人用狂犬病疫苗效价测定法(NIH)基本等效;10种疫苗随着库存时间增加疫苗效力下降.结论 M-ABT法能够成功建立,并用于灭活狂犬病疫苗效力检测效果较满意.