黄芪、赤芍及黄芪赤芍合剂对免疫性肝损伤模型小鼠血清IL-6、IL-8水平的影响

摘要

Objective To explore the influence of Astragal us, Red Peony Root and their mixture on IL-6 and IL-8 levels in models mice with immunological liver injury. Methods Bacillus Calmette-Guerin(BCG) and lipopolysaccharide(LPS) was used to construct model mice with immunological liver injury. Mice were randomly divided into normal group,model group, Astragalus group, Red Peony Root group,mixture group. No treatment was performed in normal group,and the other four groups were treated with normal saline, Astragalus extract,Red Peony Root extract and mixture,respectively. Serum IL-6 and IL-8 levels of all groups were detected. Results Serum levels of IL-6 and IL-8 were higher than those in normal group(P<0. 05) , and orderly decreased in Astragalus group,Red Peony Root group and mixture group(P<0. 05). Conclusion Astragalus and Red Peony Rood mixture could more significantly induce the decrease the serum levels of IL-6 and IL-8 in model mice with immunological liver injury than the two materials, when being used alone. To monitor the serum levels of IL-6 and IL-8 in patients, treated with Astragalus or Red Peony Root,could be helpful for rational use of drug,observation of curative effect and judgment of prognosis.%目的 观察黄芪赤芍合剂对免疫性肝损伤模型小鼠血清白细胞介素(IL)-6、IL-8水平的影响.方法 以卡介苗(BCG)和脂多糖(LPS)构建免疫性肝损伤小鼠模型.将小鼠随机分为正常组、模型组、黄芪组、赤芍组、黄芪赤芍合剂组,正常组未接受任何处理,其余各组分别经生理盐水、黄芪提取物、赤芍提取物和黄芪赤芍合剂处理后测定血清IL-6、IL-8含量.结果 模型组小鼠血清IL-6、IL-8含量高于正常组(P＜0.05),黄芪组、赤芍组及黄芪赤芍合剂组小鼠血清中IL-6、IL-8含量顺次降低(P＜0.05).结论 黄芪赤芍合剂比黄芪或赤芍单独用药能更明显地降低免疫性肝损伤小鼠模型血清IL-6、IL-8含量;监测接受黄芪或赤芍治疗的肝脏疾病患者血清IL-6、IL-8水平有助于合理用药、疗效观察及预后判断.