血清高尔基体蛋白73在肝癌高风险人群中的诊断价值

摘要

目的 定量检测肝癌、肝硬化患者及健康者血清中高尔基体蛋白73(GP73)和甲胎蛋白(AFP)含量,探讨GP73在肝癌高风险人群的早期诊断价值.方法 采用酶联免疫法(ELISA)定量检测血清GP73,采用Roche电化学发光法定量检测AFP,并对实验数据进行统计学比较(χ2检验)和ROC分析.结果 (1)在127例肝癌血清标本中,GP73的阳性率为77.2%,AFP的阳性率47.0%,经t检验,两者差异有统计学意义(χ2=24.18,P<0.01).(2)在231例非肝癌患者中,GP73的诊断特异性为95.2%(220/231),AFP的诊断特异性为83.9%(194/231);(3)ROC曲线分析显示,GP73和AFP的曲线下面积分别为0.89和0.73.结论 GP73用于肝癌患者临床检测时,显著提高了诊断肝癌的灵敏度和特异性,效果明显优于AFP.%Objective To explore the early diagnostic value of Golgi membrane protein 73(GP73) and alpha-fetoprotein (AFP) in patients with high risk of liver cancer. Methods Serum GP73 and AFP level were detected in patients with hepatic cellular cancer ( HCC) , patients with liver cirrhosis and healthy controls by enzyme-linked immunosorbent assay (KLISA) and Roche electrochemical luminescence method respectively. And the experimental data were analyzed with Chi-square test and ROC curves. Results 1. In 127 serum samples of patients with HCC,positive percentage of GP73 was 77. 2% and of AFP was 47. 0% ,and Chi-square indicated that there was significantly difference between them (x2 = 24. 18,P<0. 01). 2. In 231 cases of patients without HCC,the diagnostic specificity of GP73 was 95. 2%(220/231) and of AFP was 83. 9%(194/231). 3. ROC curve demonstrated that the area under curve (AUC) of GP73 and AFP were 0. 89 and 0. 73 respectively. Conclusion Compared with AFP,GP73 could be more sensitive and specific for the diagnosis of HCC.