免疫荧光分析法在快速诊断 A、B 型流感病毒中的临床应用

摘要

Objective To evaluate the clinical application value of Sofia Influenza A+B fIA in detecting flu virus A and flu virus B.Methods Flu virus A and flu virus B was detected in 168 samples of nasopharyneal secretions which collected from children with influenza infection by Sofia Influenza A+B FIA,GICA and RT-PCR methods.Results Sofia Influenza A+B FIA positive detection rate was 17.86%(30/168),higher than GICA method.Compared with RT-PCR,its sensitivity and specificity for flu virus A and flu virus B were 83.3%,100% and 71.4%,100% respectively.Conclusion Sofia Influenza A+B FIA is a rapid clinical application method with high sensitivity and specificity.%目的：探讨 Sofia Influenza A＋B FIA 检测系统在快速诊断 A、B 型流感病毒中的临床应用价值。方法获取临床168例流感患儿的鼻咽拭子样本，分别通过 Sofia Influenza A＋B FIA 检测系统和胶体金免疫层析（GICA）法分别进行 A、B 型流感病毒检测，并通过逆转录聚合酶链反应（RT-PCR）进行验证。结果Sofia Influenza A ＋B FIA 检测系统的阳性检出率为17．86％（30/168），高于 GICA 法；同时，与 RT-PCR 相比，对 A 型流感病毒和 B 型流感病毒的灵敏度分别达到83．3％和71．4％，特异性均达到100．0％。结论Sofia Influenza A＋B FIA 检测系统是一种灵敏度和特异性均较高，能够快速检测 A、B 型流感病毒的方法。