ANA、抗dsDNA抗体和抗ENA抗体谱对系统性红斑狼疮的诊断价值

摘要

Objective To investigate clinical value of the anti-nuclear antibody (ANA ) ,anti-double strand DNA (dsDNA ) anti-body and anti-extractable nuclear antigen(ENA) antibody repertoire in systemic lupus erythematosus (SLE) diagnosis .Methods 158 SLE patients were served as SLE group and another 50 healthy people as the control group .Indirect immunofluorescence(IIF) and immunoblotting test(IBT ) were employed to detect ANA ,anti-dsDNA antibody and anti-ENA antibody repertoire .Results Positive rates of ANA ,anti-dsDNA antibody and anti-ENA antibody repertoire in SLE diagnosis were 81 .65% ,68 .35% and 87 .34% ,respectively ,which were all lower than that of their combined detection (93 .04% ) ,with statistically significant difference (P<0 .05 ) .Among anti-ENA antibody repertoire ,the positive rate of anti-U1-nuclear ribonucleoprotein (U1-Nrnp ) antibody (52 .53% ) was the highest .Conclusion Combined detection of ANA ,anti-dsDNA antibody and anti-ENA antibodies repertoire has some clinical value of early diagnosis of SLE .%目的：探讨抗核抗体（ANA）、抗双链DNA（dsDNA）抗体及抗可溶性抗原（ENA）抗体谱对系统性红斑狼疮（SLE）的临床诊断价值。方法将158例SLE患者作为SLE组，50例健康者作为对照组。分别采用间接免疫荧光法（IIF）及免疫印迹法（IBT ）对ANA、抗dsDNA抗体及抗ENA抗体谱进行检测。结果 ANA、抗dsDNA抗体及抗ENA抗体谱检测在SLE诊断中的阳性率分别为81．65％、68．35％及87．34％，均低于三者联合检测（93．04％），差异有统计学意义（ P＜0．05）。抗EN A抗体谱中，抗U1核糖核蛋白（U1-nRNP）抗体的阳性率（52．53％）最高。结论联合检测ANA、抗dsDNA抗体及抗ENA抗体谱对SLE的早期诊断具有一定的临床价值。