目的：探讨临床生化检验中测量不确定度的评定方法及临床应用。方法使用“模式方法”评估该实验室日立7600和贝克曼DXC800两台全自动生化分析仪的13个急诊生化项目的合成不确定度和扩展不确定度，包括天门冬氨酸氨基转移酶、总蛋白、清蛋白、葡萄糖、尿素、肌酐、肌酸激酶、乳酸脱氢酶、淀粉酶、钾、钠、氯、钙。结果日立7600全自动生化分析仪检测项目中合成不确定度排前两位的是天门冬氨酸氨基转移酶和乳酸脱氢酶，分别为3．66％、3．51％；贝克曼DXC800全自动生化分析仪检测项目中合成不确定度排前两位的是钙和肌酐分别为4．00％、3．90％；两台仪器的合成不确定度比较，差值最大的是钠，为2．62％，其次是肌酐，差值为2．40％。两台仪器检测13个急诊生化项目的合成不确定度相差都比较小。结论两台仪器检测性能都比较好，“模式方法”适用于临床实验室常规检测项目的测量不确定度评估。%Objective To investigate the method and application of measurement uncertainty in clinical biochemical tests .Meth-ods The synthetic uncertainty and expanding uncertainty of 13 emergency biochemical tests items were compared between Hitachi 7600 and Beckman DXC800 automatic biochemical analyzer by using"model method".The test items included aspartate aminotrans-ferase ,total protein ,albumin ,glucose ,urea ,creatinine ,creatine kinase ,lactate dehydrogenase ,amylase ,potassium ,sodium ,calcium and chloride .Results Measurement uncertainty of aspartate aminotransferase and lactate dehydrogenase were 3 .66% and 3 .51%respectively ,which were the top two on Hitachi 7600 analyzer ;measurement uncertainty of calcium and creatinine were 4 .00% and 3 .90% respectively ,which were the top two on Beckman DXC800 .Compared between the two analyzers ,the test item with the lar-gest difference of measurement uncertainty was sodium(2 .62% ) ,and the second was creatinine(2 .40% ) .The difference of meas-urement uncertainty of the 13 emergency biochemical test items between the two analyzers were relatively small .Conclusion The two analyzer both have good performance ,"Model method"is suitable for the evaluation of regular test items′measurement uncer-tainty in clinical biochemical laboratory .
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