血小板型去白细胞滤器在浓缩血小板去白细胞中的应用

摘要

Objective To investigate the application of leukoreduction filter in removal of leukocytes in platelet concentrate . Methods Platelet concentrate was prepared by using platelet‐rich plasma method .35 bags of the same type of prepared platelet concentrate were filtered by using leukoreduction filter .The changes of conventional indicators of platelet before and after filter were measured and recorded .The activating platelets indicators PAC‐1 and CD62p were detected by using flow cytometry .The platelet hypotonic shock was measured by biochemical analyzer .The platelet aggregation was measured by using platelet aggrega‐tion instrument .Results After platelet concentrate was filtered by using leukoreduction filter ,leukocyte removal rate was(98 .28 ± 0 .97)% ,platelet recovery rate was(86 .37 ± 2 .84)% .After filtration ,white blood cell count ,platelets ,red blood cells were reduced than before filtration(P<0 .05) .The differences of capacity of platelets ,mean platelet volume and pH before and after filtration were not statistically significant(P> 0 .05) .Before and after filtration ,the expression of platelet activation markers PAC‐1 and CD62p ,platelet aggregation and platelet hypotonic shock were not statistically different(P>0 .05) .Conclusion Platelet leukore‐duction filter could effectively filter leukocytes in platelet concentrate .It would not change the conventional indicators ,and not affect platelet activation ,aggregation and anti‐hypotonic shock capacity significantly .%目的：评价血小板型去白细胞滤器在浓缩血小板中的应用效果。方法采用富血小板血浆法制备浓缩血小板，将35袋制备好的同型浓缩血小板利用血小板型去白细胞滤器过滤。记录过滤前后血小板常规指标变化情况，用流式细胞仪对血小板活化指标PAC‐1及CD62p进行检测，利用生化分析仪对血小板低渗休克进行测定，利用血小板聚集仪测定血小板聚集功能。结果利用血小板型去白细胞滤器进行过滤后，白细胞去除率为（98．28±0．97）％，血小板回收率为（86．37±2．84）％；过滤后白细胞计数、血小板、红细胞均较过滤器减少（P＜0．05），过滤前后血小板容量、平均血小板体积和pH差异均无统计学意义（P＞0．05）；过滤前后血小板活化标志物PAC‐1和CD62p表达、血小板低渗休克和血小板聚集功能差异均无统计学意义（P＞0．05）。结论血小板型去白细胞滤器能够有效滤除浓缩血小板中的白细胞，不会改变血小板常规指标，且对血小板活化、聚集功能和抗低渗休克能力均无明显影响。