Identification of Proteins Interacting at Blood or Plasma/Biomaterial Interfaces: Relation to Platelet and White Cell Adhesion, and to Clotting, and to Interactions Among White Cells and Platelets at Interfaces

摘要

This year the authors found evidence that deposition and interaction of plasma proteins depend not only on composition of the material surface, but also on temperature and on the width of spaces such as crevices. On glass, plasma deposits fibrinogen and removes it again while high molecular weight kininogen (HMWK) becomes adsorbed, unless available space is less than about 10 microns, so that platelets in these spaces will find fibrinogen still adsorbed and adhere to it. The 'critical space' (where plasma fails to contain enough HMWK per surface unit) is greater in presence of red cells since these act as a plasma diluent, so that a greater area of fibrinogen remains on glass for platelets to adhere to. Relationships between granulocyte and platelet adhesion are complicated by their preference for adsorbed globulins and fibrinogen, respectively. Platelets adhering at low temp. may induce granulocyte adhesion at higher temperatures. These factors affect evaluation of biomaterials in vitro.