目的：正确认识免疫透射比浊法应用于全自动生化分析仪上的抗原过量产生的钩状效应及对这种钩状效应的防范。方法首先对仪器的测量线性范围上限和仪器自动稀释重做功能进行设置，然后对设置前超过测量范围上限的样本进行设置测定及设置前且人工对倍稀释测定。结果设置前测定组平均值为2．49g／L设置后测定组平均值为3．31g／L设置前且人工对倍稀释测定组平均值为3．33 g／L。结论高剂量钩状效应使强阳性标本误测为弱阳性，甚至假阴性结果，高浓度标本误测为低值，所以对仪器进行设置且对标本倍比稀释来进行测定，测出来的值更接近真值。%Objective To understand correctly the hook like effect of the immune transmission and transmission turbidity in the automatic biochemical analyzer and the prevention of the hook like effect .Methods First ,it had the function set of measurement linear range of the upper limit of the instrument and the instrument automatic dilution redo ,and then ,had determination of excee‐ding the upper limit of measurement range and artificial dilution before the sample setting .Results The group average value was 2 .49 g/Lbefore the determination setting ,3 .31 g/L after the determination setting ,and 3 .33 g/L artificial dilution determination . Conclusion High dose hook effect makes the strong positive to weak positive samples of measurement by error ,even false negative results ,and high concentration sample makes low value .So the set of samples and dilution to determination of instrument ,measured values are more close to the true value .
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