Sysmex-XE5000、CellaVisionDM 96及人工显微镜分类疟疾患者外周血白细胞百分比的差异

摘要

Objective To compare the difference of peripheral blood leucocyte percentages in malaria patients among Sysmex‐XE5000 ,CellaVisionDM96 and manual microscope classification ,and to verify the accuracy and reliability of the Sysmex‐XE5000 automatic blood corpuscle detection instrument for detecting the leukocyte classification in blood routine .Methods The leucocyte percentages data in 82 cases of malaria detected by using the Sysmex‐XE5000 in the Shenzhen Municipal Third People′s Hospital from January 2011 to December 2015 were retrospectively collected ;the peripheral blood smear in 82 cases of malaria obtained the percentages after the classification by the CellaVisionDM 96 ;then the peripheral blood smear was performed the leucocyte classifica‐tion by the manual microscopy for calculating the percentage .Results In the pairwise comparison of percentage obtained from the peripheral blood leucocyte classification by Sysmex‐XE5000 ,CellaVisionDM96 and manual microscopy ,only the monocytes percent‐age had statistical difference between CellaVisionDM 96 and manual microscopy (P<0 .05) ,the other differences had no statistical significance (P>0 .05) .Conclusion By comparing the peripheral blood leucocyte percentages data in malaria patients by Sysmex‐XE5000 ,CellaVisionDM96 and manual microscopic classification ,it is indicated that the leukocyte classification data by Sysmex‐XE5000 are accurate and reliable ,malaria parasite does not affect peripheral blood leukocyte classification ,but it is necessary to pay more attention to monocytes classification in CellaVision DM 96 classification .%目的：比较疟疾患者通过Sysmex‐XE5000、CellaVisionDM 96及人工显微镜分类外周血白细胞百分比的差异性，验证Sysmex‐XE5000全自动血细胞分析仪检测疟疾患者血常规中白细胞分类结果的准确、可靠性。方法回顾性收集2011年1月至2015年12月深圳市第三人民医院收治的82例确诊疟疾患者经Sysmex‐XE5000行血常规白细胞百分比的数据；82例疟疾患者外周血涂片经CellaVisionDM 96行白细胞分类所得百分数；再经人工显微镜检82例疟疾患者外周血涂片进行白细胞分类计算后的百分数。结果 Sysmex‐XE5000、CellaVisionDM 96及人工镜检分类疟疾患者外周血白细胞百分数两两比较中，仅见Cel‐laVisionDM96组与人工显微镜组的单核细胞百分比比较差异有统计学意义（P＜0．05），其他差异均无统计学意义（P＞0．05）。结论通过比较Sysmex‐XE5000、CellaVisionDM96及人工显微镜检分类疟疾患者外周血白细胞百分比数据，表明Sysmex‐XE5000分类白细胞数据准确、可靠，疟原虫并未影响外周血白细胞分类，但在CellaVisionDM 96分类审核时需注意单核细胞分类。