鼠源性抗星形胶质细胞上调基因1单克隆荧光抗体在恶性浆膜腔积液检测中的应用

摘要

Objective To use mouse astrocytes elevated gene‐1 (AEG‐1) monoclonal fluorescent antibody for detecting tumor cells in malignant serous cavity effusions .Methods The expression of AEG‐1 in serous cavity effusion exfoliated cells by PCR and Western‐blot ;the mouse monoclonal anti‐AEG‐1 fluorescent antibody and tumor cells in malignant serous cavity effusions were co ‐incubated ,meanwhile ,the serous cavity effusions in benign lesions were taken as the negative control .Results The AEG‐1 expres‐sion was positive in malignant serous cavity effusions exfoliated cells ,while which in benign lesion serous cavity effusion was nega‐tive or weakly positive ;meanwhile the results of direct labelling in mouse anti AEG‐1 monoclonal antibodies were consistent with the results by PCR and Western‐blot .Conclusion Mouse anti AEG‐1 monoclonal fluorescence antibody can provide certain theoret‐ical basis for the detection of tumor cells in serous cavity effusion .%目的：应用鼠源性抗星形胶质细胞上调基因1（AEG‐1）单克隆荧光抗体检测恶性浆膜腔积液中的肿瘤细胞。方法应用 PCR 及 Western‐blot 方法检测浆膜腔积液脱落细胞中的 AEG‐1表达；用鼠源性抗 AEG‐1单克隆荧光抗体与恶性浆膜腔积液中的肿瘤细胞共孵育，同时采用良性病变的浆膜腔积液作为阴性对照。结果恶性浆膜腔积液脱落细胞中 AEG‐1表达呈阳性，而良性病变的浆膜腔积液脱落细胞中 AEG‐1表达为阴性或弱阳性；同时鼠源性抗 AEG‐1单克隆荧光抗体直接标记脱落细胞的结果与 PCR 和 Western‐blot 检测结果一致。结论鼠源性抗 AEG‐1单克隆荧光抗体为浆膜腔积液中的肿瘤细胞鉴定提供了新的实验手段。