目的 探讨血浆miRNAs作为食道癌早期诊断标志物的意义.方法 采集食道癌初诊患者的血浆,检测候选miR-NAs(miR-30a、miR-126a和let-7b),通过与健康对照者血浆中miRNAs表达水平比较,判断其表达是否存在差异;通过校正长期吸烟与酗酒之后,利用Logistic回归判断哪一候选miRNAs具有更好的指导临床诊断的价值;利用ROC曲线分析候选miRNAs是否具有较高的诊断效能.结果 两组人群在年龄上并无显著性差异(P>0.05),而在吸烟支数、饮酒水平及肿瘤家族史方面具有显著性差异(P<0.05).两组人群相比,血浆let-7与miR-126a与食道癌发病风险相关(P<0.05);校正吸烟与酗酒2个危险因素后,let-7b水平与食道癌发病风险相关(P<0.05).let-7b的ROC曲线下面积为0.712,其具有一定的食道癌诊断效能.结论 血浆let-7b可以作为诊断食道癌的生物标志物.%Objective To investigate diagnostic value of miRNAs for esophageal cancer .Methods Blood samples were obtained from patients with esophageal cancer .MiRNAs including miR-30a ,miR-126a ,let-7b were detected and compared with healthy con-trols .After adjusting smoking and insobriety ,Logistic regression was used to judging which was best for clinical guidance .ROC curve was used to analyzed the clinic value of these miRNAs .Results Age of the two groups had no significant difference(P>0 .05) .The levels of smoking ,alcoholism and family history of cancer in the two groups had significant difference (P<0 .05) .Two groups′miR-30a levels in plasma had no significant differences (P>0 .05) .Let-7b and miR-126a levels in plasma in patients with e-sophageal were significantly higher in healthy volunteers (P<0 .05) .After adjustment the factors of smoking and alcohol ,let-7b lev-els were associated with the risk of esophageal cancer (P<0 .05) .Area under the ROC curve was 0 .712 ,which had a certain esoph-ageal cancer diagnostic performance .Conclusion Plasma let-7b could be treated as a biomarker for the diagnosis of esophageal canc-er.
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