特发性少弱精患者精浆α-葡糖苷酶、锌与精子形态学参数相关性分析

摘要

目的 分析特发性少弱精患者精浆α-葡糖苷酶活性、锌含量与精子形态学相关参数的相关性.方法 152例特发性少弱精患者根据形态学分析分成5组,采用计算机辅助精子分析(CASA)、Diff-Quik染色法分析精液质量、精子形态,利用速率法、PAN法及全自动生化分析仪检测精浆α-葡糖苷酶活性和锌水平.结果 正常精子形态百分率在3%~4%、2%~3%、1%~2%、0%~1% 4组与正常精子形态百分率≥4%比较,精浆α-葡糖苷酶活性均有显著性差异(Z分别为-2.199、-2.685、-2.827、-3.358,P<0.05),其余各组之间比较差异均无统计学意义(P>0.05);精浆锌水平各组比较差异无统计学意义(P>0.05).精浆α-葡糖苷酶活性与正常形态精子百分率呈正相关(P<0.05),与精子畸形指数(SDI)呈负相关(P<0.05),与精子头部、颈部和中段、尾部、过量残留胞浆、多重异常指数(MAI)、畸形精子指数(TZI)无明显相关性(P>0.05);精浆锌水平与各项精子形态学参数均无相关性(P>0.05).结论 正常精子形态百分率异常的特发性少弱精患者精浆α-葡糖苷酶活性明显低于精子形态正常的患者;精浆α-葡糖苷酶活性与正常形态精子百分率呈正相关,与SDI呈负相关,可能有利于体外受精.%Objective To analyze the correlation between seminal plasma α-glucosidase activity and zinc content with sperm morphology parameters in the patients with idiopathic oligozoospermia.Methods One hundred and fifty-two cases of idiopathic oligozoospermia were divided into five groups according to morphological analysis.The quality of semen and sperm morphology were analyzed by using computer assisted sperm analysis (CASA) and Diff-Quik staining.The rate method,PAN method and automatic biochemical analyzer were used to detect the seminal plasma α-glucosidase activity and zinc content.Results The seminal plasma α-glucosidase activity had statistically significant difference between 4 groups of normal sperm morphology 3%-4%,2%-3%,1%-2% and 0%-1% with normal sperm morphology ≥4% (Z=-2.199,-2.685,-2.827,-3.358,P<0.05).There was no statistically significant difference among the other groups (P>0.05);the seminal plasma zinc content had no statistical difference among various groups (P>0.05).The seminal plasma α-glucosidase activity had a extremely significant positive correlation with percentage of normal morphology sperms (P<0.05),a significant negative correlation with sperm deformity index(SDI) (P<0.05),and had no obvious correlation with sperm head,neck,middle piece,tail,excessive residual cytoplasma,multiple abnormal index(MAI) and teratozoospermia index(TZI) (P>0.05);there was no significant correlation between the zinc content in seminal plasma with the sperm morphological parameters (P>0.05).Conclusion The seminal plasma α-glucosidase activity in idiopathic oligozoospermia patients with abnormal percentage of normal sperm mophology is significantly lower than that in the patients with normal sperm morphology;seminal plasma α glucosidase activity has significantly positive correlation with the percentage of normal mophology sperms,has a negative correlation with SDI,which may be conducive to in vitro fertilization.