Toll样受体4在妊娠期糖尿病患者外周血和胎盘组织中的表达及意义

摘要

目的 探讨妊娠期糖尿病(GDM)患者外周血和胎盘组织中Toll样受体4(TLR4)的表达及其意义.方法 选取2013年2月至2015年2月该院收治的30例妊娠期糖尿病产妇作为GDM组,以及同期体检健康的30例产妇作为健康组.采集2组产妇外周血和胎盘组织,采用RT-PCR法检测外周血单个核细胞TLR4 mRNA表达,采用免疫组化检测胎盘组织TLR4蛋白表达.结果 GDM组(0.63±0.12) 外周血单个核细胞TLR4 mRNA相对表达量明显高于健康组(0.32±0.07),差异有统计学意义(t=12.223,P<0.05).GDM组胎盘组织绒毛滋养细胞、脱膜细胞和羊膜上皮细胞中TLR4蛋白表达明显高于对照组(Z=2.325、2.374、2.162,P<0.05).结论 妊娠期糖尿病患者外周血单个核细胞,以及胎盘组织绒毛滋养细胞、脱膜细胞和羊膜上皮细胞中TLR4表达明显增强,推测TLR4可能与GDM的发病有关.%Objective To investigate the expression and significance of Toll-like receptors-4 in peripheral blood and placental tissue in gestational diabetes mellitus (GDM) patients.Methods From February 2013 to February 2015.a total of 30 cases of gestational diabetes mellitus patients(GDM group) and 30 cases of normal pregnant people(health group)were selected as research objects.Peripheral blood and placental tissue of two groups were collected.The expression of TLR4 mRNA in peripheral blood mononuclear cells was detected by RT-PCR,the expression of TLR4 protein in placenta tissue was detected by immunohistochemistry.Results The expression of TLR4 mRNA in peripheral blood mononuclear cells of the observation group was significantly higher than that of the health group[(0.63±0.12) vs.(0.32±0.07)],the difference was statistically significant(t=12.223,P<0.05).The expression of TLR4 in villous trophoblast cells,decidual cells and amniotic epithelial cells in GDM group was significantly higher than that in the health group(Z=2.325,2.374,2.162,P<0.05).Conclusion The expression of TLR4 in peripheral blood mononuclear cells,villous trophoblast cells and decidual cells in gestational diabetes mellitus patients significantly enhanced,suggesting that TLR4 might be related to the e gestational diabetes mellitus.