HIF-1 α与MUC5AC、MUC5B mRNA在慢性鼻-鼻窦炎中的表达水平与其相关性

摘要

目的 探讨缺氧诱导因子1α(HIF-1 α)与MUC5AC、MUC5B mRNA在慢性鼻-鼻窦炎(CRS)中的表达水平及其相关性.方法 选取2014年5月至2015年5月于辽宁中药大学附属医院进行治疗的80例伴鼻息肉CRS患者、80例不伴鼻息肉CRS患者以及80例无CRS健康者(对照组).收集并处理3组鼻窦黏膜,采用半定量反转录聚合酶链反应法(RT-PCR)检测HIF-1 α与MUC5AC、MUC5B mRNA的表达,并分析其相关性.结果 在伴或不伴鼻息肉CRS患者中,HIF-1 α、MUC5AC、MUC5B mRNA的相对表达量分别为:1.35±0.85、1.35±0.91,0.80±0.55、0.79±0.49,1.18±1.01、1.21±1.02,而在对照组中,它们的相对表达量分别为:0.42±0.33,0.43±0.36,0.47±0.43,CRS组与对照组比较,差异具有统计学意义(P<0.05).伴或不伴鼻息肉CRS患者HIF-1 α与MUC5AC、MUC5B mRNA均呈正相关(r=0.476、P=0.023;r=0.476、P=0.026;r=0.478、P=0.035;r=0.508、P=0.021).受试者工作特征曲线分析结果显示曲线下面积=0.956 4.结论 HIF-1 α与MUC5AC、MUC5B mRNA在CRS中的表达水平明显升高,且HIF-1 α与MUC5AC、MUC5B mRNA存在密切联系.%Objective To explore the expression levels of HIF-1 α and MUC5AC,MUC5B mRNA in chronic rhinosinusitis(CRS) and their correlation.Methods Eighty cases of CRS with nasal polyps(CRSwNP) treated in our hospital from May 2014 to May 2015,80 cases of CRS without NP(CRSsNP) and 80 healthy people without CRS(control group) were chosen.Nasal sinus mucosa in 3 groups were collected and treated.The expressions of HIF-1 α,MUC5AC and MUC5B mRNA were measured by semiquantitative reverse transcription polymerase chain reaction(RT-PCR),and their correlations were analyzed.Results The relative expression levels of HIF-1 α,MUC5AC and MUC5B mRNA in the CRSwNP group or CRSsNP group were 1.35±0.85,1.35±0.91;0.80±0.55,0.79±0.49 and 1.18±1.01,1.21±1.02 respectively,while which in the control group were 0.42±0.33,0.43±0.36 and 0.47±0.43 respectively,the difference between the CRS groups and control group was statistically significant(P<0.05).The expression of HIF-1αin the CRSwNP group and CRSsNP group was positively correlated with MUC5AC,MUC 5B mRNA(r=0.476,P=0.023,r=0.476,P=0.026,r=0.478,P=0.035,r=0.508,P=0.021).The ROC analysis results showed AUC=0.956 4.Conclusion The expression levels of HIF-1α and MUC5AC,MUC5B mRNA in chronic rhinosinusitis are significantly upregulated,moreover HIF-1α is closely related with MUC5AC and MUC5B mRNA.