胃癌患者血清生长分化因子15和胃癌抗原724检测及其临床价值探讨

摘要

Objective To investigate the change and clinical significance of growth differentiation factor 15 (GDF15) and gastric cancer antigen 724 (CA724) in serum of patients with gastric cancer .Methods Serum levels of GDF15 in serum of 30 patients with gastric cancer were detected by ELISA .The levels of serum gas-tric CA724 were measured by electrochemiluminescence ,and 32 cases of benign gastric lesions and 30 healthy controls were compared .Results The levels of serum GDF15 and CA724 in patients with gastric cancer were (1 .58 ± 0 .53)ng/mL and (40 .80 ± 5 .20) IU/mL ,respectively .The levels of serum GDF15 and CA724 in pa-tients with benign gastric lesions were (0 .26 ± 0 .11) ng/mL ,(12 .90 ± 2 .30) IU/mL (P<0 .01) .The levels of GDF15 and CA724 in normal control group were (0 .17 ± 0 .08)ng/mL and (3 .80 ± 0 .90)IU/mL respectively . The levels of serum GDF15 and CA724 in gastric cancer group were significantly higher than those in benign gastric lesions and normal control group (P<0 .01) .The level of serum GDF15 was closely related to tumor size ,TNM stage and lymph node metastasis (P<0 .05) .The sensitivity of gastric cancer was 83 .3％ ,the spe-cificity was 83 .9％ and the AUC was 0 .837 .The sensitivity of CA724 was 90 .0％ ,the specificity was 76 .5％and the AUC was 0 .886 .The combined detection of AUC was 0 .920 ,which was significantly higher than that of single detection .Conclusion GDF15 is associated with the development and progression of gastric cancer .Combined detection of GDF15 and CA724 in serum is helpful for early diagnosis of gastric cancer and progno-sis .%目的 探讨生长分化因子15(GDF15)和胃癌抗原724(CA724)在胃癌患者血清中的变化及临床意义.方法 采用双抗体夹心酶联免疫吸附试验(ELISA)检测30例胃癌患者血清中GDF15水平,同时用电化学发光法(ECL)测定血清CA724水平,并与32例胃良性病变患者和30例健康对照者进行比较.结果 胃癌组患者血清GDF15和CA724水平分别为(1.58±0.53)ng/mL、(40.80±5.20)IU/mL,胃良性病变组患者血清GDF15和CA724水平分别为(0.26±0.11)ng/mL、(12.90±2.30)IU/mL,健康对照组GDF15和CA724水平分别为(0.17±0.08)ng/mL、(3.80±0.90)IU/mL,胃癌组患者血清GDF15和CA724水平显著高于胃良性病变组和健康对照组(P<0.01).血清GDF15水平与胃癌患者肿瘤大小、TNM分期、淋巴结转移密切相关(P<0.05).GDF15诊断胃癌灵敏度为83.3％,特异度为83.9％,受试者工作特征曲线下面积(AUC)为0.837;CA724诊断胃癌的灵敏度为90.0％,特异度为76.5％,AUC为0.886.GDF15和CA724联合检测时,AUC为0.920,显著高于单项检测.结论 GDF15与胃癌的发生、发展有关,联合检测血清中GDF15和CA724有助于早期诊断胃癌,并判断患者预后.