宫颈癌患者外周血Th17、Th22、Treg及相关细胞因子变化

摘要

Objective To investigate the changes of helper T cell(Th)17,Th22,regalatory T cell regula (Treg)and related cytokines in peripheral blood of patients with cervical cancer.Methods 95 patients with cervical cancer were enrolled in the study from the hospital from January 2015 to December 2016 as the obser-vation group,meanwhile 61 patients with cervical intraepithelial neoplasia(CIN)treated in the hospital were enrolled in the study as the CIN group,and 56 healthy people who underwent healthy assessment were en-rolled as the healthy group.Peripheral blood was extracted from those people,from which peripheral blood mononuclear cells were isolated,then the percentages of Th17,Th22 and Treg cells were measured,and the se-rum levels of IL-17,IL-21 and IL-22 were measured by using enzyme-linked immunosorbent assay.Results The percentages of Th17,Th22 and Treg cells in the observation group were higher than those in the CIN group and the healthy group;the percentages of Th17,Th22 and Treg cells in the CIN group were higher than those in the healthy group,and the differences were statistically significant(P<0.05);the serum levels of IL-17,IL-21 and IL-22 in the observation group were higher than those in the CIN group and the healthy group, the serum levels of IL-17,IL-21 and IL-22 in the the CIN group were higher than those in the healthy group, and the differences were statistically significant(P<0.05);the precentages of Th17,Th22 and Treg cells in the Ⅲ - Ⅳ stage group were higher than those in Ⅰ - Ⅱ stage group,and the differences were statistically significant(P<0.05);serum levels of IL-17,IL-21 and IL-22 in the Ⅲ ~ Ⅳ stage group were higher than those in Ⅰ - Ⅱ stage group,and the differences were statistically significant(P< 0.05).Conclusion Peripheral blood Th17,Th22,Treg cells and related cytokines may play an important role in the pathogenesis of cervical cancer.%目的 探讨宫颈癌患者外周血辅助性T 细胞(T h)17、T h22、调节性 T 细胞(T reg)及相关细胞因子变化.方法 选取该院2015年1月至2016年12月收治的宫颈癌患者95例作为观察组,同期收治的宫颈上皮内瘤变(CIN)患者61例作为CIN组,另选取该院同期健康体检者56例作为健康组.纳入研究者均抽取外周静脉血,分离外周血单个核细胞,测定其中的外周血 T h17、T h22、T reg水平;采用酶联免疫吸附法测定血清白细胞介素(IL)-17、IL-21、IL-22水平.结果 观察组 T h17、T h22、T reg 水平高于CIN 组和健康组,CIN 组T h17、T h22、T reg水平表达高于健康组,差异均有统计学意义(P<0.05);观察组血清IL-17、IL-21、IL-22水平高于CIN组和健康组,CIN组血清IL-17、IL-21、IL-22水平高于健康组,差异均有统计学意义(P<0.05);Ⅲ ~Ⅳ期组 T h17、T h22、T reg水平高于Ⅰ ~ Ⅱ期组,差异均有统计学意义(P<0.05);Ⅲ ~ Ⅳ期组血清IL-17、IL-21、IL-22水平高于Ⅰ ~ Ⅱ期组,差异均有统计学意义(P<0.05).结论 外周血 T h17、T h22、T reg及相关细胞因子可能在宫颈癌发病中起着重要作用.