传染性单核细胞增多症患者不同标本EBV-DNA检测的临床价值

摘要

Objective To investigate the diagnostic value of plasma ,peripheral blood mononuclear cell (PB-MC) and throat swab EB virus (SBV) load in the patients with infectious mononucleosis (IM ) aged over 16 years old .Methods The detection results in 130 patients with suspected IM aged over 16 years old of 3 differ-ent samples of plasma ,peripheral blood mononuclear cell(PBMC) and throat swab EB virus(SBV) load were analyzed retrospectively .Results Among 61 cases of IM verified by clinic and laboratory ,16 cases of IM were detected by plasma sample ,the detection rate of EBV-DNA was 26 .22％ ;in 31 cases of PBMC detection ,the EBV-DNA detection rate was 61 .29％ ;in 31 cases of throat swab detection ,the EBV-DNA detection rate was 83 .78％ .The EBV-DNA detection rate of the combined detection with one indicator or multiple indicators positive of plasma ,PBMC and throat swab as the basis was 100 .00％ .Conclusion The combined detection of plasma ,PBMC and throat swab can improve the positive rate in the patients with IM aged over 16 years old , w hich can provide more bases for pathogenic diagnosis and treatment for clinic .%目的 探讨血浆、外周血单个核细胞(PBMC)、咽拭子的EBV-DNA载量对16岁以上传染性单核细胞增多症(IM)患者的诊断价值.方法 回顾性分析130例16岁以上疑似IM患者血浆、PBM C和咽拭子3种标本EBV-DNA载量检查结果.结果 61例临床和实验室确诊IM中,血浆标本检出16例,EBV-DNA检出率为26.22％;31例检测PBMC的患者EBV-DNA检出率为61.29％;37例咽拭子EBV-DNA检出率为83.78％.血浆、PBMC、咽拭子以一项或多项阳性为依据的联合检测,EBV-DNA检出率100.00％.结论 通过血浆、PBMC、咽拭子EBV-DNA的联合检测可提高16岁以上IM阳性检出率,为临床提供更多的病原学诊断和治疗依据.