Objective To investigate the drug resistance situation and Staphylococcal cassette chromosome mec(SCCmec) genotypes of methicillin resistant Staphylococcus aureus (M RSA ) strains isolated from Shang-hai Putuo District People′s Hospital in order to provide a theoretical basis for predicting the trend of drug re-sistant bacterial strains and clinical treatment and prevention of MRSA .Methods Three hundreds and eighty clinically isolated MRSA strains in this hospital were collected from January 2012 to December 2016 .The in vitro drug susceptibility test was performed by adopting the broth microdilution method .The SCCmec geno-types were examined by adopting the multiplex polymerase chain reaction .Results All strains were sensitive to linezolid and vancomycin ,the sensitivity rate was 100 .0％ ;the resistance rates to rifampicin and cotrimox-azole were lower ,which were 5 .0％ and 7 .6％ respectively ;but the strains were highly resistant to erythromy-cin ,levofloxacin and tetracycline ,with the resistance rate of 100 .0％ ,94 .2％ ,93 .4％ and 90 .0％ .The resist-ance rate to penicillin was 100 .0％ .Among 380 strains of MRSA ,there were 281 strains(73 .9％ ) of SCCmecⅡ ,59 strains (15 .5％ ) of SCCmecⅢand 5 strains (1 .3％ ) of SCCmecⅣa ,other 35 strains(9 .2％ ) of MRSA could not be classified .Conclusion M RSA strains isolated in the Shanghai Putuo District People′s Hospital are mainly the type SCCmecⅡ ,w hich has the multi-drug resistant characteristics ,and the drug resistance spec-trum of different SCCmec genotypes is different .%目的 了解上海市普陀区人民医院耐甲氧西林金黄色葡萄球菌(M RSA)耐药情况及SCCmec型别,为预测耐药菌株的发展趋势、为临床预防和治疗M RS A感染提供理论依据.方法 收集该院2012年1月至2016年12月临床分离的M RS A共380株,采用微量肉汤稀释法进行体外药敏试验,采用多重聚合酶链反应检测SCCmec型别.结果 所有菌株对利奈唑胺、万古霉素均敏感,敏感率为100.0％;对利福平、复方磺胺甲噁唑耐药率较低,分别为5.0％ 和7.6％;对红霉素、左氧氟沙星、四环素高度耐药,耐药率分别是100.0％ 、94.2％ 、93.4％ 和90.0％;对青霉素耐药率为100.0％.380株M RSA有SCCmecⅡ型281株(73.9％),SCC-mecⅢ型59株(15.5％),SCCmecⅣa型5株(1.3％),另有35株(9.2％)未能分型.结论 上海市普陀区人民医院分离的M RSA标本以SCCmecⅡ型为主,具有多重耐药的特征,不同SCCmec型别的耐药谱有所差异.
展开▼
