VEGF和MMP-9在大鼠视网膜缺血再灌注损伤中表达及相关性研究

摘要

目的：研究VEGF和MMP-9在大鼠视网膜缺血再灌注中的表达以及两者的相关性。　　方法：将84只健康成年Wistar大鼠随即分为正常对照组和缺血再灌注后6,12,24,48 h；3,7 d组,每组12只。采用升高眼内压的方法建立视网膜缺血再灌注模型,应用免疫组织化学法检测大鼠视网膜缺血再灌注损伤( retinal ischemia/reperfusion injury,RIRI)后视网膜组织 VEGF 和MMP-9表达的变化。　　结果：正常对照组未见VEGF阳性表达,视网膜缺血再灌注后12 h开始出现VEGF的表达,48 h达到高峰,差异有统计学意义(P<0.01)。缺血再灌注后6h MMP-9的表达开始增加,24 h 后达到高峰。正常对照组几乎未检测到MMP-9的表达,差异有统计学意义( P<0.01)。经相关性分析发现,缺血组视网膜VEGF和MMP-9的表达呈正相关(r=0.834,P<0.05)。　　结论：VEGF和MMP-9在视网膜缺血再灌注损伤中的表达呈正相关,两者可能协同作用,在视网膜缺血性病变的发生发展中起重要作用。%AIM:To investigate the expression levels and correlation of VEGF and MMP-9 in the retinal ischemia/reperfusion injury( RIRI) . METHODS: Eighty-four Wistar rats were divided into normal control group and at 6h, 12h, 24h, 48h, 3d and 7d group after retinal ischemia/reperfusion. The model of RIRI was constructed by elevating the intraocular pressure. The expression levels of VEGF and MMP-9 was measured using immunohistochemical staining. RESULTS:No VEGF positive cells were found in normal group and it began to express at 12h, increased gradually and reached maximal levels at 48h after RIRI. MMP-9 positive expression was rarely detected in the normal control group and began to increase at 6h, reached the peak at 24h. There was statistical difference between normal control group and ischemia/reperfusion group ( P<0. 01) with regard to the expression of VEGF and MMP-9. The expression of VEGF was positively correlated to MMP-9(P<0. 05). CONCLUSION:The high expression of VEGF and MMP-9 is significantly correlated with RIRI and may play an important role in the occurrence and development of retinal ischemic injury.