单面修饰疏水性人工晶状体表面细胞黏附实验研究

摘要

目的：观察乙烯基吡咯烷酮接枝前表面修饰疏水性丙烯酸酯人工晶状体植入兔眼后的表面细胞黏附的光镜改变，分析其单面修饰提高人工晶状体的生物相容性。　　方法：健康新西兰白兔13只26眼随机分组。9眼植入前表面乙烯基吡咯烷酮接枝修饰人工晶状体，8眼植入前表面二氧化钛修饰人工晶状体，9眼植入未修饰的疏水性丙烯酸酯人工晶状体。于术后90d摘除眼球并取出人工晶状体进行光镜分析。　　结果：单面乙烯基吡咯烷酮接枝修饰后的人工晶状体表面黏附细胞数量和面积、蛋白膜的形成均低于对照组。　　结论：疏水性人工晶状体经乙烯基吡咯烷酮接枝单面修饰后可明显提高人工晶状体的葡萄膜生物相容性。%AIM:To assess the biocompatibility of hydrophobic acrylic intraocular lenses after anterior surface modification with monomer vinyl pyrrolidone by studying cell adhesion using light microscopy. METHODS:Twenty-six eyes of 13 rabbits were divided into 3 groups randomly.Nine eyes were implanted into vinyl pyrrolidone modification intraocular lenses, 8 eyes were implanted into titanium oxide modification intraocular lenses, 9 eyes were implanted into unmodified intraocular lenses following lens extraction. The intraocular lenses implanted eyes were enucleated in 90d of post operation and cell adhesion on surface of different intraocular lenses were compared under light microscopy. RESULTS:The cells'size, number and protein films of intraocular lenses on anterior surface of vinyl pyrrolidone modification group were significantly less than that in two control groups. CONCLUSION: The uveal biocompatibility of hydrophobic acrylic intraocular lenses after single surface modification by monomer vinyl pyrrolidone is significantly improved.