眼底荧光素血管造影在小鼠氧诱导视网膜病变中的应用评价

摘要

目的：：在小鼠氧诱导视网膜病变模型( oxygen-induced retinopathy,OIR )中评价眼底荧光素血管造影( fundus fluorescein angiography,FFA)的应用价值。方法：将前期实验证实视网膜病变严重程度有明显差异的两组( B组>A组)各12只新生幼鼠于出生后第7 d置于75％浓度氧环境中,第12 d时返回正常空气环境中饲养。第17 d时将A组和B组的幼鼠均随机分配,分别进行FFA检查或高分子量FITC-Dextran灌注结合视网膜铺片检查,即每种检查方法纳入两组幼鼠各6只,利用图像分析软件对视网膜无灌注区进行定量分析和比较。结果：FFA结合图像分析软件能对视网膜无灌注区进行定量分析,与FITC-Dextran灌注结合视网膜铺片的测量结果有良好的可比性,两种方法得到的结果无统计学差异(P>0.05)。结论：FFA结合图像定量分析在小鼠OIR模型的血管病变评价中具有一定的实用价值。%AIM:To investigate avascuIopathy-evaIuating method using fundus fIuorescein angiography ( FFA) in mice with oxygen-induced retinopathy ( OIR) . METHODS:OIR modeI was induced by exposure of mice from groups A and B ( the retinopathy was known to be more severe in group B than that in group A by previous research) to high oxygen (75%) from postnataI day 7 (P7) to P12 (n=12 for each group) and returned to normaI environment at P12. On P17, the mice from both groups were randomIy assigned to accept FFA or high moIecuIar weight fIuorescein isothiocyanate dextran ( FITC - Dextran ) perfusion combined with stretched preparation of retina( each method invoIved 6 pups from each group ) . The retinaI non - perfused areas were quantified and compared by using image anaIysis software.RESULTS:FFA combined with image anaIysis software was abIe to quantify the retinaI non-perfused areas, which was comparabIe to the resuIts anaIyzed by FITC-Dextran perfusion combined with stretched preparation of retina. No statisticaI difference was found between the resuIts obtained by the two methods (P>0. 05). CONCLUSION:FFA combined with image quantification anaIysis can be used in retinaIvascuIopathy evaIuation in mouse OIR modeI.