针对微流控电泳芯片检测系统微型化、集成化的要求,分析了传统电泳芯片检测系统的优势和不足,提出一种以FPGA芯片为控制器的CCD多通道微流控电泳芯片检测系统.利用FPGA/NiosⅡ嵌入式系统解决方案,以EP2C8Q208芯片为核心,设计了CCD驱动及外围硬件电路.通过上位机软件进行数据处理,实现了荧光图谱的同步显示.实验结果表明:该系统能同时检测多通道微流控电泳芯片中各通道不同的荧光信号强度,具有较高的灵敏度和信噪比,对罗丹明B样品的最低检测浓度为1.0×10-6mol/L,能够满足多通道微流控电泳芯片检测的要求.%Based on the requirement with detection system for microfluidic electrophoresis chip in terms of miniaturization, integration , the traditional equipment advantage and the shortage was analyzed. A CCD detection system for multi-channel microfluidic electrophoresis chip used FPGA as the main logic controller was proposed. FPGA/Nios Ⅱ embedded system was adopted into the project which designed the CCD driving and other periphery circuit with the EP2C8Q208 chip as its core. The data was transferred to the PC. Then the fluorescence spectrum was shown by the software. The result indicated that the system can detect the different fluorescence intensity in the micirofluidic chip at the same time. The minimum detecting limit is 1.0 × 10-6 mol/L (Rhodamine B). This detecting system has several characteristics such as high signal-noise ratio and high sensitivity, which can meet the demand of detection of the multi-channel micirofluidic electrophoresis chip.
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