The three new full-length cDNA sequences including the complete 5'-and 3'- untranslated regions (UTR) coding for cytochrome P450s from Aedes albopictus have been obtained. The P450 proteins deduced from the nucleotide sequences shared 58.6% - 62.4% amino acid identity with CYP6N1 and CYP6N2 from Anopheles gambiae, and 99% with each other. The three new complete sequences have been submitted and named as CYP6N3v1,CYP6N3v2 and CYP6N3v3 by the P450 Nomenclature Committee. The original cDNAs were obtained by rapid amplification of cDNA ends (RACE) approach with several pairs of gene specific primers based on the cDNA fragment previously obtained from deltamethrin-resistant strain of Ae. albopictus. Further analysis showed that the three new sequences are present in both resistant strain and susceptible strain and might be effectively translated. In addition, the 5' - and 3' -UTRs were compared between the CYP6N3v1-v3 and other known insect P450s. The multiplicity of translational control of insect P450 genes was discussed.%获得白纹伊蚊细胞色素P450 3个新全长cDNA序列及其5'-和3'-非翻译区序列.所推导的3个P450蛋白与来自冈比亚按蚊的CYP6N1、CYP6N2的氨基酸同源性为58.6%-62.4%,相互之间的同源性则高达99%.该3个全长cDNA序列已报国际P450命名委员会,被正式命名为CYP6N3v1、CYP6N3v2和CYP6N3v3.它们是根据本文作者最近从白纹伊蚊溴氰菊酯抗性株中所获得的一个CYP6基因cDNA片段设计了3对基因特异性引物用cDNA末端快速扩增(RACE)方法而获得的.进一步分析显示:该3个新的cDNA序列同时存在于白纹伊蚊溴氰菊酯抗性株及敏感株中,并能进行有效地翻译.另外,还比较了CYP6N3v1-v3与其它已知的昆虫P450的5'-及3'-非翻译区的结构,讨论了昆虫P450翻译水平调控的复杂性.
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