KL－6黏蛋白和E－钙黏蛋白在大肠癌组织中的表达及临床意义

摘要

Objective Research the relationship between the expression of KL -6 mucin and E-cadherin and the clinical and pathological features in colorectal cancer tissue .Methods Collect 6 0 cases colorectal cancer tissue and normal tissue adjacent cancer when surgery (require surgical patients without preoperative systemic chemotherapy ,radiation therapy ,and other treatment ) ,using reverse transcriptase polymerase chain reaction technology to detect the changes of KL -6 mucin and E -cadherin gene from RNA in colorectal cancer tissues and normal tissue adjacent cancer .Results(1 )Respectively with ELISA method to detect :the expression of KL-6 mucin in colorectal cancer tissues is obviously higher than normal tissue adjacent to cancer ,the expression of E-cadherin in colorectal cancer tissues is obviously lower than normal tissue adjacent to cancer ,the difference was statistically significant ( P<0 .0 5 ) .The expression of KL -6 mucin and E -cadherin in colorectal cancer was related to degree of cell differentiation tumor and lymph node metastsis ( P>0 .0 5 ) ,but not to age ,Sex , TNM Stages (P<0 .05 ) .There was impositive correlation between KL -6 mucin and E -cadherin expression in colorectal cancer tissue(P<0 .05 ) .Conclusion The excessive expression of KL -6 mucin and E -cadherin in colorectal cancer play an important role in tumor ’s development ,KL -6 mucin and E-cadherin e expression in colorectal cancer has correlation with differentiation degree and the lymph node metastasis United detection the expression of KL -6 mucin and E-cadherin colorectal cancer may help to evaluate the malignant degree and the judgment about prognosis .%目的：研究KL －6黏蛋白和E－钙黏蛋白在结直肠癌中的表达和其对应的临床病理之间相互关系。方法于手术中收集大肠癌组织与癌旁组织各60例（所有手术患者在术前均未进行全身化疗、放疗及其他治疗），采用逆转录酶聚合酶链反应技术检测大肠癌组织及癌旁组织中KL －6黏蛋白与E－钙黏蛋白的RNA表达水平的变化。结果（1）ELISA法检测结果显示：大肠癌组织中KL －6黏蛋白的表达明显高于癌旁正常组织，而E－钙黏蛋白表达明显低于癌旁正常组织，差异具有统计学意义（P＜0．05）。KL －6黏蛋白和E－钙黏蛋白的表达与性别、年龄、临床TNM 分期无关，且差异无统计学意义（P＞0．05），但与不同肿瘤的分化程度以及有无淋巴结转移有关，且差异有统计学意义（P＜0．05）。（2）相关性分析显示KL －6黏蛋白和E－钙黏蛋白在大肠癌中的表达呈显著负相关（ P＜0．05）。结论（1）K L －6黏蛋白和E －钙黏蛋白的表达异常在大肠癌的发生、发展中起重要作用，KL －6黏蛋白和E－钙黏蛋白的表达与大肠癌的分化程度及淋巴结转移情况有相关性；联合检测两者在大肠癌中的表达可能有助于恶性程度的评估与预后判断。（2）KL －6黏蛋白其发挥作用的作用机制可能与其抗粘附作用以及Wnt／β一catenin信号通路的激活密切相关。