为了研究黑玫瑰石竹组培快繁中激素等其他因子的影响，我们设计了一系列含不同生长素（NAA）和细胞分裂素（6-BA、KT）浓度配比，不同种类及浓度的碳源（蔗糖、白砂糖和麦芽糖），不同大量元素（MS、1/2MS、1/4MS和2MS）和不同添加物（水解乳蛋白、谷氨酰胺、椰汁和活性炭）的培养基来培养黑玫瑰石竹离体茎段。研究结果表明：NAA/6-BA为0.1 mg/L/1.0 mg/L的浓度配比的培养基适合黑玫瑰石竹试管苗的快速繁殖，KT对试管苗的生长及根的发生影响显著；组培快繁中适宜黑玫瑰石竹生长的最佳碳源是15 g/L的蔗糖，也可用30 g/L的白砂糖代替蔗糖，以降低成本；最适宜茎芽增殖的培养基是MS培养基，同时试验得出添加谷氨酰胺后芽的增值系数可高达9.4，比其他几种添加物的影响均显著。%In order to study the effect of different factors on Dianthus Linn tissue culture and rapid propagation , we had designed a series of culture medium which containing different concentration proportions of growth hormone (NAA) and cytokinin (6-BA,KT), different specious and concentration of carbon source (sugar,white granulated sugar and maltose), different major element (MS,1/2MS,1/4MS and 2MS) and different supplement ( Lactalbumin Hydrolysate,glutamine, coconut juice and activated carbon) to culture it's isolated stem segments. The results showed that when the concentration of NAA and 6-BA was 0.1mg/L/1.0mg/L, the medium suitable for explants' rapid propagation, while KT had significant difference in plantlet growth and root elongation. In plant tissue culture and rapid propagation , the best carbon source was sugar of 15 g/L and also would be replaced by sugar of 30 g/L to cost reduction. MS medium was fit for micro shoot multiplication, and at the same time, in the experiment the bud's multiplication coefficient was reach up to 9.4 after adding glutamine, which was significant than other supplements.
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