北寄生提取物抑制人肝癌HepG2细胞增殖及诱导凋亡的实验研究

摘要

目的:探讨北寄生提取物对人肝癌HepG2细胞增殖抑制和凋亡诱导作用.方法:分别采用噻唑蓝(MTT)法检测细胞增殖活性、光镜观察细胞结构变化、流式细胞仪检测细胞凋亡.结果:0.3mg·mL-1北寄生提取物处理24h后,细胞增殖明显受到抑制,且抑制率随时间延长而增加;光镜下可观察到细胞凋亡形态的改变;0.3mg·mL-1北寄生提取物处理48h后,流式细胞仪检测细胞凋亡率为24.7%.结论:北寄生提取物不仅能有效地抑制肿瘤细胞的增殖,同时又有诱导肿瘤细胞凋亡的作用.%Objective:To explore the anti -proliferation and apoptosis -induced effects of Viscum coloratum extracts(VCE) on HepG2 cells.Methods: HepG2 cell proliferative activity was measured by MTr assay.Cell apoptosis was observed by Giemsa staining method.Cell apoptosis rate was determined by flow cytometry.Results :After exposure to VCE at concentrations 0.3mg· mL-1 for 24h, cellular proliferation was inhibited with the increase of the concentration and the time.The significant apoptotic morphology changes were observed.After exposure to VCE at concentrations 0.3mg·mL-1 for 48h, cell apoptosis was determined as 24.7％.Conclusion:VCE can not only inhibit the proliferation, but also induce apoptosis in HepG2 cells.