桦菌芝多糖对S180瘤组织p53蛋白表达的影响

摘要

To study the effect on p53 protein expression in S180 tumor tissue with Pyropolyporus Fomentaries polysaccharide (PFPS) and inhibition of sarcoma - S180 cell proliferation of mice. Methods; The tumor weight was measured to calculate the tumor inhibition rate. Immunohistochemistry method was used to detect p53 protein in tumor tissue treated with PFPS. Results; The PFPS treatment at 100 and 50mg/kg resulted in tumor growth inhibition rates of 41. 84% and 41. 13%. Immunohistochemistry analysis demonstrated the expression level of mutatant p53 decreased. There was a significant difference compared with the negative control group(P <0.05). Conclusion; The PFPS has an inhibitory effect against S180 sarcoma in vivo and can decrease the expression level of mutatant p53.%目的:观察桦茵芝多糖对小鼠移植型肉瘤(S180)的抑制作用及对瘤组织p53蛋白表达的影响.方法:建立S180小鼠动物模型,取瘤组织称其质量,计算抑瘤率；用链霉菌抗生素蛋白:过氧化酶免疫组化法来测定瘤组织中p53蛋白含量,通过多媒体图像分析系统分析实验结果.结果:桦茵芝多糖高、中剂量组抑瘤率分别为41.84％、41.13％,桦茵芝多糖高、中剂量组S180瘤组织突变型p53蛋白表达减少,与模型组比较差异显著(P＜0.05).结论:桦茵芝多糖对小鼠肉瘤S180有抑制作用,可下调小鼠S180瘤组织突变型p53基因表达.