熟地黄多糖诱导 H22荷瘤小鼠细胞凋亡作用的研究

摘要

Objective:To investigate RPS vivo anti-tumor mechanism of induction of apoptosis of tumor cells, the impact of Bcl-2 protein expression and apoptotic morphological changes,and to provide the basis for clini-cal application.Methods:The H22 ascites mice were inoculated with tumor strain, and the preparation models were divided into the saline group,the cyclophosphamide group and the RPS group.Immunohistochemistry was used to detect tumor tissue Bcl-2 protein expression and apoptosis by TUNEL index.Results:Compared with the model group,Bcl-2 protein mean optical density in the RPS group was lower,and the difference was sig-nificant(P<0.01).The apoptotic index in the RPS group increased significantly(P<0.05).Conclusion:RPS can inhibit the H22 tumor tissue expression of Bcl-2 in mice,so the number of apoptotic cells increases signifi-cantly,and RPS can effectively induce apoptosis in tumor-bearing mice.%目的：探讨熟地黄多糖体内诱导肿瘤细胞凋亡的作用机理，观察熟地黄多糖对肿瘤细胞中Bcl－2蛋白表达的影响及细胞凋亡形态学上的变化，为临床应用提供实验依据。方法：采用H22肝癌腹水瘤株接种小鼠制备模型，随机分为模型组、阳性对照组、熟地黄多糖组。免疫组化法检测肿瘤组织中Bcl－2蛋白表达、TUNEL法检测细胞凋亡指数。结果：与模型组比较，熟地黄多糖组的Bcl－2蛋白平均光密度值降低，与模型组比较差异显著（P＜0．01）；熟地黄多糖组的凋亡指数明显上升（P＜0．05）。结论：熟地黄多糖对H22荷瘤小鼠肿瘤组织中Bcl－2蛋白的表达有明显的抑制作用，使凋亡细胞数目明显增多，熟地黄多糖能有效诱导H22荷瘤小鼠肿瘤细胞发生凋亡。