雷公藤内酯对海人酸活化的BV－2小胶质细胞Ras和 Raf表达的影响

摘要

目的：观察雷公藤内酯对海人活化的BV－2小胶质细胞Ras、Raf蛋白及mRNA表达的影响，揭示雷公藤内酯抑制海人酸活化的BV－2小胶质细胞增殖的分子机制。方法：免疫组化法检测BV－2小胶质细胞Ras和Raf蛋白表达，RT－PCR法检测BV－2小胶质细胞Ras和Raf mRNA表达。结果：免疫组化结果显示，海人酸组BV－2小胶质细胞Ras和Raf蛋白表达与对照组相比显著增加（ P＜0．05），雷公藤内酯组BV－2小胶质细胞Ras和Raf蛋白表达与海人酸组相比显著降低（ P＜0．05）。 RT－PCR结果显示，海人酸组BV－2小胶质细胞Ras和Raf mRNA表达水平与对照组相比显著增加（P＜0．05），雷公藤内酯组BV－2小胶质细胞Ras和Raf mRNA表达水平与海人酸组相比显著降低（P＜0．05）。结论：雷公藤内酯可能通过下调BV－2小胶质细胞Ras、Raf蛋白及mRNA表达抑制海人酸活化的BV－2小胶质细胞增殖。%Objective:To observe the effect of triptolide on the expressions of Ras and Raf in kainic acid-acti-vated BV-2 microglial cells and reveal the molecular mechanism of triptolide for inhibiting the proliferation of BV-2 microglial cells.Methods:Immunohistochemistry technique was uesd to detect the expressions of Ras and Raf protein in BV-2 microglial cells.RT-PCR was used to detect the expressions of Ras and Raf mR-NA in BV-2 microglial cells.Results: Immunohistochemistry technique showed that the expressions of Ras and Raf protein in BV-2 microglial cells in kainic acid group increased significantly compared with those in the control group (P<0.05).However, the expressions of Ras and Raf protein in BV-2 microglial cells in triptolid group decreased significantly compared with those in the kainic acid group (P<0.05).The results of RT-PCR showed that the expressions of Ras and Raf mRNA in BV-2 microglial cells in kainic acid group increased significantly compared with those in the control group (P<0.05).However, the expressions of Ras and Raf mRNA in BV-2 microglial cells in triptolid group decreased significantly compared with those in the kainic acid group ( P<0 .05 ) .Conclusion:Triptolide can suppress the proliferation of kainic acid-activated BV-2 microglial cells by downregulating the expressions of Ras and Raf protein and mRNA.