产絮凝剂微杆菌的絮凝特性及印染废水处理应用

摘要

An efficiently flocculant-producing bacterial strain WX-7 has been screened out and obtained as Micro-bacterium sp.,through 16s rDNA determination. It grows best under the following conditions:pH is 8.0,inoculum concentration 1%,temperature 30℃,and rotational speed 170 r/min,by adding sucrose and dried corn steep liquor powder and culturing for 16-24 h. The flocculating activity of Strain WX-7 mainly distributes on thallus. The best coagulant aid is Ca2+. When pH is higher than 11,and 3 mL of bacterial liquid,and 3 mL of CaCl2 solution (1%) are added to kaolin suspension(0.5%),the maximum flocculating activity can reach 98%. The flocculant ingredient is not sensitive about heat treatment. The flocculant produced from Strain WX-7 can effectively remove the chroma and COD from dyeing wastewater. The decolorization rate and COD removing rate are 81%and 42%,respectively. It indicates that this strain has certain application prospect in wastewater treatment fields.%筛选获得一株高效产絮凝剂菌株WX－7，通过16s rDNA鉴定，该菌株隶属于微杆菌属。在pH为8．0、接种量1%、30℃、170 r/min条件下，添加蔗糖、玉米浆干粉，培养16~24 h生长最佳。 WX－7菌株的絮凝活性主要分布于菌体，最佳助凝剂为Ca2+。在pH>11条件下，向0．5%的高岭土悬液投加3 mL菌液和3 mL 1%的CaCl2溶液，絮凝活性最高可达98%。絮凝剂成分对热处理不敏感。 WX－7菌株所产絮凝剂能有效去除印染废水的色度和COD，脱色率和COD去除率分别为81%、42%，在废水处理中具有一定的应用前景。