本文从不同年龄、不同地域的银杏叶、茎、根部组织中分离得到20株银杏内共生真菌，经过发酵复筛有5株的产黄酮能力超过5μg／mL。实验对菌株的最佳发酵产黄酮条件进行了优化，优化条件为：3％葡萄糖、0．5％蛋白胨、0．4％酵母膏、0．3％ KH2 PO4、0．01％ MgSO4·7H2 O；起始pH值7 ．0、最适发酵温度28℃、摇床转速为140 r／min、装液量125 mL／250 mL。在此条件下，该系列菌株在发酵7 d左右产黄酮量可达6 ．4 μg／mL。%20 strains were obtained from endophytic fungi,which were separated from leaves,stems and roots of Ginkgo biloba L. with different ages and from different areas of Hefei. After fermentation and screening,five strains of them had more than 5 μg/mL flavonoid-producing capability. And then,the flavonoid-producing conditions for these strains were optimized as follows:3% glucose,0. 5% peptone,0. 4% yeast extract,0. 3% KH2 PO4 ,0. 01% MgSO4 ·7H2 O;initial pH 7. 0,optimum fermentation temperature 28 ℃,shaker speed 140 r/min,liquid volume in flask 125 mL/250 mL. Under the optimized conditions,this fungus could produce as high as 6. 4 μg/mL flavonoid after incubation for 7 days.
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