选择性检测谷胱甘肽的荧光探针

摘要

本文设计合成了一种基于BODIPY衍生物选择性检测谷胱甘肽的比率式荧光探针1.荧光探针1中BODIPY的3位连有苯乙炔基团,5位连有咪唑盐离去基团,利用其与谷胱甘肽和半胱氨酸反应机理的不同实现了对谷胱甘肽的选择性检测.紫外可见吸收光谱和荧光光谱实验结果表明探针分子1与谷胱甘肽反应后的光谱发生明显红移,可以实现对谷胱甘肽的比率式检测.探针分子1对谷胱甘肽有极高的选择性,不受其它氨基酸尤其是半胱氨酸的干扰.荧光滴定实验表明探针分子1可实现对谷胱甘肽的定量检测,检测限为3.3×10-8 mol/L.探针分子成功地应用于活体细胞中检测谷胱甘肽.%We developed a ratiometric fluorescent probe based on BODIPY which can detect GSH selectively.We did UV-Vis and fluorescence experiments and found that the UV-Vis and fluorescence spectra of probe 1 with GSH exhibited obvious red shifts.Probe 1 exhibited high selectivity and the detection inhibited the interference of Cys and was not interfered by other amino acids.The fluorescence titration experiments showed that probe 1 realized the quantitative detection of GSH with detection limit of 3.3 × 10 8 mol/L.The probe was successfully applied to the detection of GSH in living cells.