亚麻下胚轴对卡那霉素及膦化麦黄酮的敏感浓度筛选

摘要

The influence of different concentrations of Kanamycin (Kan) and phosphinothricin (PPT ) on the differentiation and growth of Linum usitatissimum L. hypocotyl was studied using L. usitatissimum cultivars L. usitatissimum cv. Pax, L. usitatissimum cv. Zhongya No.2 and L. usitatissimum cv. Huaxing 009 as materials on the basis of efficient regeneration system. Results showed that 50 mg/L Kan or 1.5 mg/L PPT could totally inhibit differentiation of L. usitatissimum hypocoyl in early selection. It was the appropriate concentration.%在亚麻(Linum usitatissimum L.)高效再生体系建立的基础上,以亚麻栽培品种派克斯(L.usitatissimum cv.Pax)、中亚2号(L.usitatissimum cv.Zhongya No.2)和华星009(L.usitatissimum cv.Huaxing 009)为材料,研究了不同浓度的卡那霉素(Kanamycin,Kan)和膦化麦黄酮(Phosphinothricin,PPT)对亚麻下胚轴分化和生长的影响.结果表明,在亚麻遗传转化早期选择时,50 mg/L的Kan或1.5 mg/L的PPT能够完全抑制亚麻下胚轴的分化,是适宜的筛选浓度.