The effect of in vitro competition on shoot regeneration from hypocotyl explants of Linum usitatissimum

摘要

This study was carried out to investigate the relationship between in vitro competition and stress, and their effects on tissue culture response of Linum usitatissimum L. hypocotyl explains. Competition among explants was achieved by varying the spacing among the explants cultured. Four different culture spacing distances were used: 0.5, 1.0, 1.5, and 2.0 cm. Six weeks after culture initiation, hypocotyl fresh and dry weights, shoot regeneration percentage, shoot number per hypocotyl, regenerated shoot length, total shoot number per petri dish, and total chlorophyll content were recorded. The results showed that encouraging competition among explains by decreasing spacing among them from 2.0 cm to 1.0 cm increased shoot number per hypocotyl, regenerated shoot length, and total shoot number per petri dish in both cultivars. When explants were cultured at 0.5 cm spacing, significant stress-initiated decreases were observed in all parameters examined. This study showed that the success of tissue culture studies for related genotype could be increased not only by determination of correct concentrations and combinations of auxins and cytokinins in growth medium but also by evaluating competition among explants cultured.