为了研究银杏叶提取物(GBE)对由硫代乙酰胺(TAA)所致肝损伤大鼠肝脏的保护作用及其对Caspase-8的影响,并探讨其作用机制.将大鼠分为5组,每组10只,GBE低、中、高剂量组(50、100、200mg/kg)连续灌服GBE 30 d,1次/d,正常对照组和模型组分别给予等体积的生理盐水；模型组、GBE低、中、高剂量组第28天用TAA(300 mg/kg)腹腔注射,正常对照组腹腔注射等体积的生理盐水.结果表明,模型组与正常对照组相比,血清中谷丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)水平升高,肝组织中Caspase-8在mRNA水平上调表达；而GBE组与模型组相比,血清中ALT、AST水平降低,肝组织中Caspase-8在mRNA水平下调表达.GBE可通过调节Caspase-8的表达抑制急性肝衰竭肝细胞调亡,从而对肝脏起到保护作用.%50 rats were divided into Eve groups and treated with different levels uf ginkgo biloba extract (GBE) to study the protection effects of GBE on TAA-induced acute liver failure (ALF) rats, its effect on liver Caspase-8 and the function mechanism. The three treat groups were successively administrated for 30 days with low, middle and high dose of GBE, such as 50, 100 and 200mg/kg, respectively. The control group and the model group were administrated with saline at the same volume. At the 28th day, the model group and three treat groups were treated with thioacetamide (TAA) of 300mg/kg by in-traperitoneal injection. The control group was treated with saline. The results showed that compared with the control group, the serum alanine aminotransferase (ALT) and aspartate transaminase (AST) of the rats in the model group were obviously increased, and Caspase-8 mRNA in liver was overexpressed. Compared with the model group, the levels of serum ALT and AST of the mice in GBE treat groups were significantly decreased and the gene expression of Caspase-8 was significantly down regulated in liver. It indicated that the protective effects of GBE on liver were realized through regulating the expression of Caspase-8 to inhibit the hepatic apoptosis in acute liver failure rats.
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