空心莲子草不同溶剂提取液的抑菌活性研究

摘要

With ethanol,petroleum ether,acetone and acetic ether as solvents to extract the active ingredient of Alternanthera philoxeroides (Mart.)Griseb,the antibacterial effects were tested by the inhibitory zone method,and minimum inhibitory concentration was tested by the dilution method.The results showed that Alternanthera philoxeroides (Mart.) Griseb extracts had better antibacterial effects.The minimum inhibitory concentrations of ethanol extracts and petroleum ether extracts against Aspergillus niger and ethanol extracts against oral candidiasis were all 0.25％.Ethanol extracts had stronger antimicrobial activity than other solvents.The results indicated that antibacterial activities of extracts were affected by solvents.Besides,by testing antibacterial activities of ethanol extracts under different temperature,it was found that the extracts had better heat stability.%以乙醇、石油醚、丙酮、乙酸乙酯作为溶剂对空心莲子草全草有效成分进行提取,采用抑菌圈法比较提取液对6种受试茵的抑茵作用,用稀释法测定对受试茵的最小抑茵浓度.结果表明,空心莲子草提取液有较强的抑茵活性,乙醇、石油醚提取液对黑曲霉的最小抑菌浓度为0.25％,乙醇提取液对口腔念珠茵的最小抑菌浓度为0.25％,乙醇提取液抑茵活性总体好于其他溶剂提取液,溶剂影响提取液的抑茵能力.不同温度处理后乙醇提取液的抑茵活性测试表明提取液具有较好的热稳定性.