茶叶生产废料中表没食子儿茶素没食子酸酯的纯化工艺

摘要

An orthogonal test was used to optimize the extraction technology of EGCG from tea production waste,and to sepa-rate and purify the Epigallocatechin-3-gallate by macroporous resin.The results showed the optimal conditions for separation and purification were: D101 macroporous resin, the concentration of sample was 20.0 mg/mL, the volume of sample was 4.5 times of column volume, flow rate was 1.5 BV/h 20% ethanol for elution, elution velocity was 0.5 BV/h while elution vol-ume was 4.0 BV.%分析了茶叶生产废料中表没食子儿茶素没食子酸酯(EGCG)的纯化工艺,并采用大孔树脂对其进行分离纯化. 结果表明,运用静态吸附与动态吸附相结合的方法分析得到,适用于茶叶生产废料中EGCG分离纯化用的大孔吸附树脂为D101. 其最佳分离纯化条件为: 将20.0 mg/mL上柱液以1.5 BV/h上柱4.5 BV,使用20%乙醇以0.5 BV/h洗脱4.0 BV.