无子刺梨组织培养和快速繁殖技术研究

摘要

以无子刺梨(Rosa sterilis)去叶片及皮刺的健壮枝条为外植体，通过对从诱导到组培苗生根各步的反复试验，摸索总结出无子刺梨组织培养和快速繁殖各步的最佳配方。结果表明，最佳初代诱导培养基为LM+1.0 mg/L KT+0.5 mg/L BA+0.02 mg/L TDZ+0.05 mg/L NAA；最佳增殖培养基为LM+1.0 mg/L KT+1.0 mg/L BA+0.02 mg/L TDZ+0.05 mg/L NAA；最佳生根培养基为LM+0.1 mg/L NAA。%Using strong branches that leaves and prickles were removed off as explant, tissue culture of seedless Rosa sterilis was carried out to explore its suitable medium from induction to rhizogenesis stage. The results showed that the suitable medium of initial induction was LM medium with 1.0 mg/L KT, 0.5 mg/L BA,0.02 mg/L TDZ and 0.05 mg/L NAA; the best proliferation occurred on LM medium with 1.0 mg/L KT, 1.0 mg/L BA,0.02 mg/L TDZ and 0.05 mg/L NAA; and the best rooting condition for plantlets was on LM medium with 0.1 mg/L NAA.