不同产地花脸香蘑菌丝体及子实体的RAPD分析

摘要

Using RAPD analysis of the five different regions of mycelia and fruiting body of Lepista sordida DNA diversity. Respectively with 12 primers for PCR amplification, five samples selected by 8 primers' amplification result for data analysis, the genetic similarity coefficient between the sample value(GS),and then according to the genetic similarity coefficient matrix, the cluster analysis using UPGMA method. The results showed that RAPD mycelia and fruiting body Lepista sordida method is divided into two groups, and the mycelium of three different regions can be divided into two subsets, it can be seen that the genetic relationship provide reference for later study of Lepista sordida.%采用RAPD分析了5个不同产地的花脸香蘑(Lepista sordida)菌丝体和子实体的DNA多样性.用12种引物分别对5种样品进行PCR扩增,从中选出8种引物扩增后的结果进行数据分析,计算样品间的遗传相似系数(GS值),进而根据遗传相似系数矩阵,利用UPGMA法进行聚类分析.结果表明,花脸香蘑菌丝体和子实体分为两个类群,又将3个不同产地的菌丝体分为2个亚群,为花脸香蘑后期研究提供参考.