Dlmo基因编码一个32 kDa的蛋白

摘要

In order to gain some insight into a possible function of Dlmo gene，we used the TNT coupled reticulocyte lysate systems as an in vitro translation system to detect the Drosoplila protein. We found a 32kDa protein product.To demonstrate that the DLMO protein has the same size in vivo as in vitro we studied nuclear and cytoplasmic extracts from 2-4h embryos in Western blots. The immuno serum recognizes a 32kDa protein in the cytoplasm that is not present in the nucleus.The products were immune precipitated with the polyclonal anti-LMO2 antibody raised against the human protein and seen a positive band of 32kDa.Using immuno histochemical analysis was seen a positive staining in the basal cytoplasm of blastoderm embryos at 4 hours. This result confirms that the DLMO is a cytoplasmprotein, not like human LMO protein.%为了获取研究Dlmo（果蝇LMO基因的简称）的功能信息，使用耦联网织红细胞体外翻译系统将Dlmo基因进行体外转录翻译得到32 kDa的蛋白产物。该蛋白产物与抗人类LMO2蛋白的多抗抗体发生免疫沉淀，并得到了32 kDa的阳性带。为了证实Dlmo基因在体外和体内翻译能得到同一大小蛋白，从2～4小时果蝇胚盘中分别抽提核和胞浆提取物进行Western印迹分析，免疫血清可以识别胞浆提取物中的32kDa蛋白，而在核提取物中未曾见到，证实体外和体内翻译产物相同。免疫组织化学的分析在0～4小时胚盘外周胞浆中有Dlmo基因的阳性染色信号，结果证实，Dlmo与人类LMO不同，其表达产物是一个胞浆蛋白。