肌肉增强因子2(Myocyte enhancer factor 2,MEF2)是MADS(MCM1,agamous,deficiens和serum response factor)家族成员之一,在动物发育过程中起到重要的调节作用.为了进一步了解其调控的复杂性,本文根据NCBI中已有的人类MEF2相关数据,应用ExPASy在线序列分析工具、CBS在线分析服务器软件、Conserved Domain Database(CDD)数据库、SABLE在线分析软件等对人类MEF2蛋白的不同亚型序列进行比较分析,同时,根据相关序列的比对结果构建系统进化树进行分析.结果表明,MEF2在人体内以多种蛋白形式存在,其理化性质存在一定差别,可能的翻译后糖基化修饰多为O型糖基化且均存在较多磷酸化位点.人类各MEF2蛋白具明显MADS结构域,多数具有MEF2结构域和HJURP_C结构域.各MEF2蛋白二级结构均包括了螺旋、折叠和无规则卷曲等多种形式,其三级结构模式相似.系统进化树显示MEF2B蛋白与其他蛋白有着较大的序列差异及较远进化关系,可能较为原始.%As one of the members of MADS family, MEF2 group is important in regulating development. Analytical tools of NCBI, ExPASy, CBS, CDD, and SABLE were adopted to analyze the properties of human MEF2 proteins, and evolutionary tree was built according to the result of correlative sequence alignments. The results showed that there are various forms of MEF2 in human body, and there are some differences in the physicochemical characteristics. Relatively more phosphorylation sites are found and the main glycosylation sites are N-glycosylation sites. All MEF2 proteins of human contain MADS domain, and most contain MEF2 domain and HJURP_C domain. Their secondary structures contain three dominant states: helix, sheet and coil, their tertiary structures are similar. The phylogenetic tree result shows that MEF2B may be original because of its difference of sequences and evolutional relation.
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