水稻'三明'显性核不育基因的定位

摘要

The mutant of "Sanming Dominant Genie Male Sterile Rice" was found from an F2 population of cross "SE21S/Basmati370" by Sanming Institute of Agricultural Science in 2001. It has proven that the male sterility of this mutant is controlled by a dominant gene (named as SMS). By multiple backcrosses, this dominant male sterile allele was introduced into the genetic background of an indica rice cultivar Jiafuzhan (which was known as Jiabuyu). In order to map SMS, a mapping population was constructed by crossing Jiabuyu with a japonica cultivar Nipponbare and further crossing the F1 with Jiafuzhan. By bulked segregant analysis and linkage analysis using SSR and INDEL markers, SMS was mapped to a 99 kb interval between INDEL markers ZM30 and ZM9 on chromosome 8. This result will facilitate cloning of SMS.%"三明显性核不育水稻"突变体是由福建省三明市农业科学研究所于2001 年在杂交组合"SE21S/Basmati370 "的F2 代群体中发现的.其不育性受1 个显性基因控制(将该基因命名为SMS).经过多代回交,该显性不育基因已导入籼稻品种佳福占的遗传背景中(将该不育材料称为佳不育).为了定位SMS,文章将佳不育与粳稻品种日本晴杂交,并将F1 与佳福占测交,构建了一个作图群体.利用SSR 和INDEL 标记,通过混合分离分析和连锁分析,将SMS 定位于第8 号染色体上两个INDEL 标记ZM30 和ZM9 之间,约99 kb 的区间内.该结果为克隆SMS 奠定了基础.