鱼藤素对斑马鱼胚胎COX/EP信号通路相关基因表达的影响

摘要

目的 研究鱼藤素斑马鱼胚胎COX/EP信号通路相关基因表达的影响.方法 采用实时荧光定量核酸扩增检测系统(real-time quantitative PCR detecting system,qPCR),比较鱼藤素作用前后目的 基因表达水平的变化.结果 鱼藤素处理组COX/EP信号通路上的ptges、ptges3a、ep4a、ep4b、ep2a、ep2b、egr1、cyclin D1基因表达水平分别下降到空白对照组的0.46±0.16,0.82±0.06,0.59±0.03,0.12±0.03,0.15±0.04,0.16±0.01,0.19±0.08,0.33±0.06,均差异有统计学意义(P＜0.01或P＜0.05).结论 鱼藤素作用的斑马鱼胚胎在sphere期停止发育,其机制可能与鱼藤素抑制COX/EP信号通路上主要基因的表达相关.%0bjective To study the effect of deguelin on genes expression in COX/ EP pathway of zebrafish embryos. Methods Real-time quantitative PCR detecting system was used to compare genes expression pre- and post- treatment of deguelin. Results Compared with the control, the expression on transcript levels of ptges, ptges3 a, ep4 a, ep4b, ep2 a, ep2b, egr1, cyclinDl in COX/EP pathway were decreased to 0.46±0.16,0. 82±0.06,0. 59±0.03,0. 12±0.03,0.15 ±0.04, 0.16±0.01,0.19±0.08,0.33±0.06, respectively(P＜0. 01 or P ＜0.05). Conclusion Deguelin arrests zebafish embryonic development at sphere stage, which may be related to the inhibition of main genes expression in the COX/ EP signaling pathway