目的 建立槲皮素固体脂质纳米粒的制备工艺与含量测定方法.方法 以山嵛酸甘油酯、胆固醇、大豆卵磷脂为载体材料,丙酮-三氯甲烷(1:1)为有机相,以泊洛沙姆、聚乙二醇、聚山梨酯80、纯化水为水相,乳化温度75 ℃,搅拌器转速600 r·min-1,冰水固化温度2 ℃进行制备;采用高速离心-高效液相色谱法测定槲皮素含量,色谱柱:Diamonsil-C18柱(250 mm×4.6 mm,5 μm);流动相:甲醇-4.3%乙酸溶液(55:45),流速1.0 mL·min-1;检测波长254 nm,进样量20 μL,柱温30 ℃.结果 制得固体脂质纳米粒稳定,槲皮素在2.0～200.0 μg·mL-1范围内线性关系良好,A=1.343 5C-1.490 4(r =0.999 6),平均回收率为97.7%,RSD=1.09%(n =6).结论 所建制备工艺稳定,含量测定方法 便捷,可靠,准确,可行.%Objective To establish a preparatian process and content determination method for quercetin solid lipid nanoparticles( QUE-SLNs ). Methods The oil phase for preparing QUE-SLNs was mainly made of Glyceryl behenate ( ATO ),soy lecithin and cholesterol, and the aqueous phase was made of poloxamer, PEG-DPSE and Tween 80. The temperature for emulsion was set at 75 ℃ , for solification from ice to water was at 2 ℃ ,and the agitator for emulsion was 600 r · min-1. The quercetin was determinated by a high-velocity centrifugation and HPLC. The Diamonsil C18( 250 mm×4.6 mm,5μm ) was used, methanol-acetic acid ( 55∶ 45 ) was taken as mobile phase at 1.0 mL · min -1 , column temperature was 30 ℃ and the sample was loaded with 20 μL. Results QUE-SLNs were stable and presented as linear relation at the range of 20.0 ～ 200.0 μg · mL 1 ,A= 1. 343 5Cl. 490 4( r= 0. 999 6 ). The average recovery was 97.7％ and RSD was 1.09％ ( n = 6 ). Conclusion Peparation process for QUE-LCL is stable and content determination is simple, reliable, accuracy, and feasibility.
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