鄂西北地区不同生长条件和品种柴胡中多糖含量比较

摘要

目的 测定鄂西北地区不同生长条件和品种柴胡中多糖的含量.方法 采用蒽酮-硫酸比色法,以620 nm波长为检测波长,分别测定柴胡中可溶性多糖和粗多糖,计算总多糖含量.结果 鄂西北地区野生竹叶柴胡多糖含量高于同地区种植柴胡,但低于山西产北柴胡和湖北南漳种植的三岛柴胡.结论 不同生长条件的柴胡药材中柴胡多糖含量不同,但差异不明显;不同品种柴胡药材中的多糖含量有明显差异.%Objective To compare the content of polysaccharide in different species of Bupleurum under different growth conditions in Northwest area of Hubei Province. Methods The contents of polysaccharide in Bupleurum was determinated by colorimetric method and detected at 620 nm. Results Polysaccharide content in wild Bupleurum marginatum Wall, ex DC. was higher than that in the planted Bupleurum in Northwest Area of Hubei Province, but lower than that in Bupleurum chinese DC. of Shanxi Province and that in B. falcatum L. of Hubei Nanzhang. Conclusion Polysaccharide content of Bupleurum Radix Bupleurum is different under different growth conditions, but no significant difference between the polysaccharide content in different varieties of Bupleurum Radix