高良姜素对衰老小鼠模型学习记忆的影响

摘要

目的 探讨高良姜素对D-半乳糖致衰老小鼠学习记忆能力的改善作用.方法 皮下注射D-半乳糖制备小鼠衰老模型.用Morris水迷宫和电跳台法测试小鼠的学习记忆能力,用生物化学方法测定大脑海马超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量.结果 与正常对照组比较,模型组学习记忆能力减退,大脑海马SOD、GPx、CAT活性降低,MDA含量增高.与模型组比较,高良姜素高、低剂量组均能显著改善衰老小鼠学习记忆能力,提高小鼠海马内SOD、GPx、CAT活性,降低MDA含量.结论 高良姜素能显著改善D-半乳糖致衰老小鼠学习记忆能力,提高大脑海马区SOD、GPx、CAT活性,降低MDA的含量,具有较好的抗氧化能力.%Objective To investigate the effect of galangin ( GL) on cognitive impairment in the senescent mice induced by D-galactose (D-gal). Methods O-gal was subcutaneously injected to establish a senescent mice model. The Morris water maze test and step-down avoidance one were performed to study the learning and memory abilities of mice. The activities of superoxide dismutase ( SOD ) , glutathione peroxidase ( GPx) , catalase ( CAT) and content of malondialdehyde ( MDA) in mice hippocampus were detected by biochemical examination. Results The mice treated with D-gal generated significant cognitive deficiency. Administration of GL to O-gal treated mice ameliorated cognitive impairment in the hippocampus. Biochemical examination revealed that GL attenuated the decreased activities of SOD, GPx, CAT and reduced MDA levels in the hippocampus of D-gal-treated mice. Conclusion Galangin possesses anti-oxidative effect by improving the cognitive function, and increasing activities of SOD, GPx, CAT and lowering MDA of the senescent mice induced by D-gal.c