复方“前愈”总黄酮部位的分离纯化与含量测定

摘要

目的 从中药复方“前愈”中提取分离总黄酮化学部位,建立总黄酮及淫羊藿苷的含量测定方法.方法 采用溶剂法结合聚酰胺树脂柱层析法分离纯化总黄酮部位；应用紫外分光光度法和高效液相色谱法,分别测定分离提取液及纯化液中总黄酮及淫羊藿苷的含量.结果 溶剂法提取分离所得总黄酮含量8.86％,淫羊藿苷含量为0.14％；纯化后总黄酮含量为28.36％,淫羊藿苷含量为3.19％.结论 该分离纯化方法操作简便合理,可显著提高前愈复方中总黄酮及淫羊藿苷含量.%Objective To extract and separate total flavonoids from the Chinese herbal compound qianyu and establish a method for determination of total flavonoids and icariin. Methods The solvent combined with the polyamide resin column chromatography was adopted to separate and purify the total flavonoids. The content of total flavonoids and icariin in the extracts was determined by ultraviolet spectrophotometry and HPLC. Results The contents of total flavonoids and icariine were 8.86% and 0. 14% after extraction,and were 28. 36% and 3. 19% after purification, respectively. Conclusion This extraction and purification approach is simple and reasonable, which can evidently improve the content of total flavonoids and icariin extracted from compound qianyu.