熊果酸对慢性非细菌性前列腺炎模型大鼠的治疗作用

摘要

Objective To investigate the therapeutic effect and mechanism of ursolic acid on carrageenan-induced chronic nonbacterial prostatitis (CNP) of SD rats. Methods Forty-two SD rats were randomly divided into five groups:normal control group, model control group, Pushitai group, ursolic acid high-dose group, and low-dose group.Except for normal control group, chronic nonbacterial prostatitis model was established using carrageenan. The rats of Pushitai group were treated with the solution of Pushitai at the dose of 148 mg��kg-1��d-1 for twenty days.The rats of ursolic acid high-dose and low-dose groups were administered with ursolic acid at the doses of 100 and 50 mg��kg-1��d-1 for twenty days, respectively. The rats of normal and model control groups were treated with isometric 5% CMC-Na for twenty days.The prostate index (PI) was calculated from the ratio of isolated rat prostate weight and body weight of rat.The expressions of IL-1β, TNF-α, and IL-10 were detected by ELISA. The histology and morphology of the prostate was observed with the aid of hematoxylin-eosin staining. Results Compared with the model control group, PI and the levels of IL-1β, TNF-α, and IL-10 in serum were significantly decreased in ursolic acid high-dose,and low dose groups ( P<0. 05 ) . Moreover, the pathological changes of prostate were improved significantly. Conclusion Ursolic acid has certain therapy effects on chronic nonbacterial prostatitis, which may be related to its regulating the expressions of IL-1β, TNF-α, and IL-10.%目的：探讨熊果酸对角叉菜胶诱导的慢性非细菌性前列腺炎( CNP )大鼠的治疗作用及其机制。方法将42只斯泼累格��多雷( SD)大鼠随机分为正常对照组、模型对照组、普适泰组、熊果酸大剂量组和小剂量组，除正常对照组外，其他组造CNP模型。普适泰组灌胃给予普适泰药液148 mg��kg－1��d－1，熊果酸大剂量组和小剂量组分别灌胃给予熊果酸药液100和50 mg��kg－1��d－1，正常对照组和模型对照组灌胃给予等体积0.5％羧甲基纤维素钠溶液，连续给药20 d。考察大鼠的前列腺指数，利用酶联免疫吸附测定( ELISA)检测大鼠血清中白细胞介素-1β( IL-1β)、肿瘤坏死因子-α( TNF-α)和白细胞介素-10( IL-10)的水平，经苏木精-伊红( HE)染色观察前列腺组织的病理学变化。结果与模型对照组比较，熊果酸大剂量组、小剂量组大鼠前列腺指数显著降低(P<0.05)，血液中细胞因子IL-1β、TNF-α和IL-10的水平下降(P<0.05或P<0.01)。前列腺组织切片HE染色显示熊果酸大剂量组前列腺炎病理组织学改变有明显的改善。结论熊果酸对角叉菜胶诱导的慢性非细菌性前列腺炎大鼠具有一定的治疗作用，其机制可能与熊果酸对IL-1β、TNF-α和IL-10水平的调节作用有关。