琥珀消石颗粒对肾结石模型大鼠肾组织病理改变及血中炎症因子的影响

摘要

目的：观察琥珀消石颗粒对肾结石模型大鼠肾组织病理改变及血中炎症因子白细胞介素(IL)-1β、肿瘤坏死因子(TNF)-α及 IL-6的影响。方法 SD 大鼠60只，正常对照组10只给予纯化水灌胃，其余大鼠给予乙二醇、钙片和维生素 D3灌胃4周，制备肾结石动物模型。将造模成功的大鼠随机分为模型对照组、枸橼酸钾组和琥珀消石颗粒小、中、大剂量(3.335，6.670，13.340 g•kg－1)组，每组10只，分别灌胃给予相应试药，4周后，肾组织切片苏木精-伊红(HE)染色，观察各组肾组织病理及含钙结石的变化。采用酶联免疫吸附(ELISA)法检测大鼠血中 IL-1β、TNF-α及 IL-6水平。结果与模型对照组比较，琥珀消石颗粒小、中、大剂量组肾间质未见出血，肾组织中可见少数或未见含钙结晶沉积。正常对照组、模型对照组、枸橼酸钾组和琥珀消石颗粒小、中、大剂量组血中 IL-1β含量分别为(50.50±13.54)，(104.00±24.13)，(93.00±20.08)，(77.00±25.21)，(71.30±26.60)，(65.50±26.18) pg•mL－1；IL-6含量分别为(570.10±119.74)，(1040.00±260.15)，(861.40±130.04)，(740.00±161.31)，(680.00±239.49)，(640.00±238.42) pg•mL－1；TNF-α含量分别为(470.30±154.63)，(1010.00±230.94)，(820.00±192.12)，(830.50±110.01)，(720.00±140.28)，(715.00±173.54) pg•mL－1；与模型对照组比较，琥珀消石颗粒小、中、大剂量组3项指标均显著降低(P<0.05或 P<0.01)。结论琥珀消石颗粒能抑制肾结石模型大鼠肾组织病理改变，抑制肾组织含钙结晶沉积，降低血中炎症细胞因子水平。%Objective To investigate the effect of Hupo Xiaoshi granules on renal tissue pathological changes and IL-1β, TNF-α and IL-6 in blood of nephrolith model rats. Methods Sixty SD rats were divided into normal control group 10 rats with purified water by intragastric administration(IG),and model group 50 rats with ethylene glycol, calcium and vitamin D3 by IG for 4 weeks to get kidney stones animal models. The successful model rats were randomly divided into model control group, potassium citrate solution group, Hupo Xiaoshi granules low, middle and high dose group (3. 335,6. 670,13. 340 g • kg-1 , respectively), 10 rats per group with the corresponding drugs. Four weeks later, rat's renal tissue sections were stained with hematoxylin and eosin, renal tissue changes of calcium stones and pathology were observed.Plasma concentrations of IL-1β, TNF-α and IL-6 were detected by ELISA. Results Compared with the model control group, no renal interstitial hemorrhage and a few or no visible renal tissue calcium crystal deposition in the low, middle and high dose Hupo Xiaoshi granules group. In the normal control group, model control group, potassium citrate solution group, and low, middle and high dose Hupo Xiaoshi granules group,the plasma concentrations of IL-1β were respectively(50.50±13.54),(104.00±24.13),(93.00±20.08),(77.00± 25.21),(71.30 ±26.60),(65.50±26.18) pg•mL-1 ,respectively;those of IL-6 were(570.10±119.74),(1 040.00±260.15), (861.40±130.04),(740.00± 161. 31),(680. 00 ± 239. 49),(640. 00 ± 238. 42) pg•mL-1 ,respectively;and TNF-α plasma concentrations were(470.30± 154. 63),(1 010. 00 ± 230. 94),(820. 00 ± 192. 12),(830. 50 ± 110. 01),(720. 00 ± 140. 28), (715.00±173.54) pg•mL-1 ,respectively. Compared with the model control group, the plasma concentrations of IL-1β, IL-6 , TNF-α of the low, middle and high dose Hupo Xiaoshi granules group were significantly decreased (P<0.05 or P<0.01). Conclusion Hupo Xiaoshi granules can inhibit renal tissue pathological changes of nephrolith model rats, inhibit calcium crystals stones deposition in renal tissue, and reduce blood levels of inflammatory cytokines.